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Cpf cas site

2019-05-09 · One such feature is the ability of Cpf1 to make staggered cuts whereas Cas9 makes blunt cuts in the genome. Cas9 makes a DSB in target site by cutting each strand in opposite directions. These cleavages result in two double stranded ends. The nuclease active site of Cpf1 cuts target ssDNA in cis and the non-target ssDNA in transposition. With the ability to recognize T-rich PAM sites, Cas12a further expands the number of available target sites for genome editing.

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There is a growing number of Cas-based strategies to address limitations of Cas9 in achieving specific and efficient genome editing. Cpf1, also known as Cas12 is used for its properties in targeting AT-rich regions for which Cas9 is less specific. Cpf1’s preferred PAM is 5’-TTN, differing from that of Cas9 (3’-NGG) in both distribution and GC-content, which might improve editing in AT-rich genomes.

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EC: 270-325-2. CAS: 68424-85- 3. SAMMANSÄTTNING/INFORMATION OM BESTÅNDSDELAR. Sammansättning enligt CLP (Förordning 1272/2008/EC).

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Hämtningar: 90; Listor: 0 Se necessário, verificar sua senha de acesso no site da ACREDITO R20/21/22 – Farligt vid inandning, hudkontakt och fortäring. 3. SAMMANSÄTTNING/UPPGIFTER OM BESTÅNDSDELAR. Ämne. CAS-nr. EG-nr.